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Global Proteomics: Protein Profiling, Characterization and Identification Services Proteomics is a a large-scale and comprehensive study of proteins, which are vital parts of any living organism’s functioning, including protein abundances, variations and modifications. In proteomics discovery, pretome analysis can be done in two different methods, the bottom-up method and top-down method. In bottom-up approach, there is protease digestion of a crude protein mixture, then liquid chromatography separation is done, followed by MS analysis, whereas top-down approach involves MS characterization of protein without prior proteolysis is done, that provides greater sequence coverage, prevent sequence ambiguities and PTMs preservation. Proteomics offer different services such as mass spectometry (electrospray ionization, matrix assisted laser desorption, ioan trap MS, etc.), peptide fractionation (strong cation exchange, ion-pairing reversed phase, weak anion exchange, etc.), protein fractionation (isoelectric focusing, cell surface labeling, affinity depletion, etc.), sample extraction (biopsy, biofluid, laser capture microdissection, primary cell culture, etc.), protein quantification (metabolic labeling, chemical protein labeling, enzymatic peptide labeling, etc.), and protein identification (database searching, de novo sequencing, peptide mass fingerprint, accurate mass and time tag, etc.). Most protein profiling projects today involves quantification of protein samples relative to another protein sample of in absolute terms. Protein profiling now use data-dependent and data-independent methods. In data-dependent technique, it involves the selection of peptide fragments for subsequent MS/MS analysis depending on pre-determined criteria, whereas in data-independent technique, it involves fragmentation of all peptides indiscriminately, with a pre-established mass range. Although data-independent methods are relatively new, there are promising results for the future of quantitative proteomics, because it is potentially able to analyze all species present in a specific sample at concentrations which are detectable, using a single assay, and the data can be used as improved software, as well as silico databases become available. Proteomics research is used in many scientific fields such as cancer research, diabetes research, discovery of diagnostic biomaker, plant and animal phenotyping, microbiology, drug discovery and drug development, toxicological testing, and study and treatment of disease through antibody profiling. Proteomics companies offer different proteomics services including protein gel and imaging analysis, protein identification, protein quantification, top-down proteomics, peptidomics service, protein post-translational modification analysis, protein-protein interaction and many more. Protein and peptide identification, with the advancement of mass spectometry techonology, have shortened time duration, which enhances the accuracy of protein sequencing.
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A trusted, reliable and experienced proteomics company can assist you in your scientific research through their wide-range protemics services that include molecular weight determination, molecular weight distribution of peptides and proteins service, sample preparation, digestion service, peptides or protein purity analysis, peptide mass fingerprinting (PMF), peptides and proteins sequence analysis, de novo protein sequence analysis, shotgun protein identification, accurate mass determination, membrane proteomics and subcellular proteomics service.5 Uses For Education